Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs
A recent publication in Nature Plants from Ellison et al. (2020) titled "Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs" describes an editing approach with Cas9. The mutant progeny recovered in the next generation had a frequency of 65 to 100% Read the article. Read the CBS press release.
TurboID-Based Proximity Labeling for In Plant Identification of Protein-Protein Interaction Networks
The article "TurboID-Based Proximity Labeling for In Plant Identification of Protein-Protein Interaction Networks" by Zhang et al. (2020) describes a proximity labeling method for identification of interaction partners of the TIR domain of the NLR immune receptor in tobacco. Read the article.
Agrobacterium tumefaciens: A Bacterium Primed for Synthetic Biology
The recently published "Agrobacterium tumefaciens: A Bacterium Primed for Synthetic Biology" by Thompson et al. reviews plant engineering using A. tumefaciens and describes how it could be further exploited for use in the transformation of a wider range of plants. Read the article.
Identification of Differential, Health-related Compounds in Chardonnay Marc through Network-Based Meta-Analysis
The article by Simmons et al. "Identification of Differential, Health-related Compounds in Chardonnay Marc through Network-Based Meta-Analysis" published in Current Developments in Nutrition used publicly available datasets to investigate the differences in different grape varieties to determine their differences using a network-based meta-analysis. Read the article.
Retip: Retention Time Prediction for Compound Annotation in Untargeted Metabolomics
A recent publication in Analytical Chemistry by Bonini et al. titled "Retip: Retention Time Prediction for Compound Annotation in Untargeted Metabolomics" highlights the issue of unidentified peaks in LC-MS/MS. The authors used machine learning and publicly available datasets to predict retention times. Read the article.
A systems analysis of E. coli short and long-term transcriptomic response to biocides
The recently published "A systems analysis of E. coli short and long-term transcriptomic response to biocides" by Pereira et al. aimed to identify the genetic basis of biocide stress response in E. coli to determine the potential mechanisms of tolerance to biocides. Read the article.
FRS7 and FRS12 recruit NINJA to regulate expression of glucosinolate biosynthesis
A recent publication in Europe PMC by Fernandez-Calvo et al. titled "FRS7 and FRS12 recruit NINJA to regulate expression of glucosinolate biosynthesis genes" identified two transcriptional repressors that accumulate during short-day conditions in Arabidopsis. Read the article.
Plant NLR immune receptor Tm2 activation requires NB-ARC domain-mediated self-association of CC domain
Wang et al.'s recent publication in PLOS Pathogens aimed to discover the mechanism of NLR immune response in plants. The authors found a signaling domain for plasma membrane-localized NLR. Read the article.