from https://academic.oup.com/plphys/advance-article/doi/10.1093/plphys/kiac159/6564672

Recent Publication: High-efficiency multiplex biallelic heritable editing in Arabidopsis using an RNA virus

A recent publication in Plant Physiology by Nagalakshmi et al. (2002) titled "High-efficiency multiplex biallelic heritable editing in Arabidopsis using an RNA virus" described the rapid adoption of genome editing technology for gene function studies in plants. Instead of the common use of Agrobacterium-mediated transformation, the authors detail new optimization of a Tobacco rattle virus-based vector for the delivery of single-guide RNAs. This allows for efficient multiplexing of somatic and biallelic editing in a single generation. 

 

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