Bits & Bites # 03: Skyline: Getting Started with Targeted Metabolomics Data Processing

Instructor: Dr. Christopher Ashwood, Protea Glycosciences

• Required Software: Skyline (https://skyline.ms/skyline64.url)
• Participant prerequisites: Basic understanding of LC-MS and method design (targeted and untargeted methods)
• Level: Intermediate
• Short description of the course: Skyline is a freely available, vendor-neutral software that facilitates targeted qualitative and quantitative analysis of both protein and small-molecule mass spectrometry data. Using hands-on tutorials, we will provide best practices on analyzing metabolomics LC-MS data with Skyline including iterative and sensitive targeted method development, small molecule target confirmation at both the MS1 and MS/MS levels, and analyte quantification using internal calibrants and external calibration curves.