The Biotechnology Programs 2008 Summer Short Courses:

Flow Cytometry July 14 - 18

Flow cytometry is a powerful tool in modern biology. The applications are numerous, and have contributed to many fields including by virology, immunology, and molecular biology. This course begins with introductory flow cytometry and progresses through development and design of multicolor panels. Afternoon sessions will include hands-on training in applications and training on various instruments. Students will be exposed to the Cytomation MoFlo cell sorter, Becton Dickinson FACScan flow cytometer, Coulter XL-MCL flow cytometers and Compucyte laser scanning cytometer, and others. Lead Instructors: Carol Oxford, Director, UC Davis Optical Biology Lab; Susan DeMaggio, President and CEO, FloCyte Associates, Inc.; Barbara L. Shacklett, PhD, Assistant Professor, Dept. of Medical Microbiology and Immunology, School of Medicine, UC Davis. Register at: http://www.cevs.ucdavis.edu/Flow

Advanced PCR Techniques: Quantitative Real-Time PCR July 28 August 1

This very popular course covers sampling strategies to obtain high quality sample material, micro-dissection of frozen or paraffin embedded tissues using the new state-of the art PALM Laser Catapult (Zeiss, PALM Microlaser Technologies), sample preparation and nucleic acid recovery from tissue, blood and paraffin samples, quality controls for sample preparation, and real-time TaqMan PCR and contamination control and monitoring. Students can work on their own samples, including micro-dissection of frozen or paraffin embedded tissues, RNA extraction, QC and gene profiling. Lead Instructor: Emir Hodzic, DVM, PhD, Director of Lucy Whittier Molecular & Diagnostic Core Facility, UC Davis. Register at: http://www.cevs.ucdavis.edu/advancedpcr

Proteomics: Fundamentals and Technology Platform August 25 29

This introductory proteome analysis workshop is designed to expose students to fundamental technology platforms and current information in the field of proteomics. The course will cover both 2-D gel based proteomics and liquid chromatography (LC) based multidimensional protein identification technology (Mudpit) employing a complex mixture of proteins. Students will also perform a database search with MS data generated in the process of protein identification. Lectures will cover: fundamentals of protein chemistry and mass spectrometry (MS), MS based protein identification, posttranslational modification of proteins, database search using MS data, and traditional tools of protein biochemistry. Lead Instructor: Brett Phinney, PhD, (UC Davis Proteomics Facility, UC Davis Genome Center). Register at:http://www.cevs.ucdavis.edu/Cofred/Public/Pro/ConfHome.cfm?confid=356

DNA Microarrays: Theory, Techniques and Analysis September 8 12

This hands-on course is a must for any researcher who wants explore the global analysis of gene expression through DNA microarray analysis. Lectures will be presented by industry scientists as well as academic researchers on the basics of chip technology, including analysis and quality control, and application of this technology in a wide range of research fields. The laboratory sessions will include preparation of cDNA from RNA, DNA hybridization to and processing of the microchip. Computer laboratory sessions will include data analysis with clustering as well as other bioinformatics tools. Lead Instructor: Satya Dandekar, PhD, UCDavis School of Medicine, Katrin Stapleton, PhD, Affymetrix Register at: http://www.cevs.ucdavis.edu/dna

For more information on all courses, go to http://www.biotech.ucdavis.edu/ and click on Courses or call (530)752-3260.