Primers for Eberhardy, S.E. and Farnham, P.J., 2002

Primers used for cloning Myc fusion constructs

GST-Myc Primers

Primer Name	Sequence
BH Myc 1	5’-CATGCGATCGGATCCATGCCCCTCAACGTTAGC-3’
BH Myc 36	5’-CATGCGATCGGATCCCAGCAGAGCGAGCTGCAG-3’
BH Myc 41	5’-CATGCGATCGGATCCTCACTGCAGCTCGCTCTGCTGCT-3’
BH Myc 70	5’-CATGCGATCGGATCCTCAGCAGAGCCCGGAGCGGCGGCT-3’
BH Myc 103	5’-CATGCGATCGGATCCTCAGGTCACCATCTCCAGCTGGTC-3’
BH Myc 120	5’-CATGCGATCGGATCCTCACGGGTCGCAGATGAAACTC-3’
BH Myc 143	5’-CATGCGATCGGATCCTCAGGCGGCGGCCGAGAAGCCGCT-3’

Gal4-Myc primers

Primer Name	Sequence
Sc Myc 1	5’-CATGCGATCGAGCTCATGCCCCTCAACGTTAGC-3’
Sc Myc 36	5’-CATGCGATCGAGCTCCAGCAGAGCGAGCTGCAG-3’
Sc Myc 70	5’-CATGCGATCGAGCTCTCAGCAGAGCCCGGAGCGGCGGCT-3’
Sc Myc 120	5’- CATGCGATCGAGCTCTCACGGGTCGCAGATGAAACTC-3’

 

Bold sequences indicate restriction enzyme recognition sites
BH Myc 1, BH Myc 36, Sc Myc 1 and Sc Myc 36 are 5’ primers; the rest are 3’ primers