CURRICULUM VITAE

Name:            Charles Meyer Nicolet

Born:             January 9, 1958
                        Providence, Rhode Island

Address:       UC Davis Genome Center
                        Room 4212
                        Genome and Biomedical Sciences Facility
                        One Shields Ave
                        Davis, CA 95616

Telephone:      Cell: (530) 304-1163;  lab: (530) 754-5281

Education:      1980-1986      Graduate Student
                                    Department of Genetics
                                    Stanford University
                                    Stanford, CA  94305
                                    PhD, Genetics

                         1976-1980      Undergraduate
                                    Bowdoin College
                                    Brunswick, ME  04011
                                    B.A., Biology  Magna cum lauda

Research Experience:

July 2004-Present:  Facility Manager, DNA Technologies Core, and Facilities Manager, Expression Analysis Core of the UC Davis Genome Center

October 1997-July 2004: Facility Director, DNA Sequence and Synthesis Facility at the University of Wisconsin Biotechnology Center.

July 1996-October 1997: Associate Scientist in Dr. Paul Sondel's lab, assessing the use of biolistic ("gene gun") technology to deliver genes encoding therapeutically promising antibody-cytokine molecules in murine pre-clinical models.

December 1993-June 1996: Assistant Scientist in Dr. Paul Sondel ’s lab in the Dept. of Human Oncology, working on development of chimeric antibody-cytokine molecules for use as immunotherapeutics. 

June 1993-December 1993:  Assistant Scientist in Dr. Donna Paulnock's lab. 

October 1990- May 1993:  Postdoctoral fellow in Dr. Donna Paulnock's lab, analyzing the molecular responses of macrophages to interferon-gamma.

January 1987-October 1990:  Postdoctoral fellow in Dr. Elizabeth Craig's lab, undertaking a biochemical and genetic analysis of the function of hsp70 proteins in Saccharomyces cerevisiae.

June 1986-December 1986:  Postdoctoral fellow in Dr. Errol Friedberg's lab, continuing my graduate work on investigation of the activities of the Rad2 protein.

June 1984-June 1986:  Graduate student in the laboratory of Dr. Errol Friedberg in the Stanford University Medical School.  Characterization of the RAD2 gene from Saccharomyces cerevisiae, encoding a protein involved in DNA excision repair. 

March 1981-June 1984:  Graduate student in Dr. A.T. Ganesan's laboratory in the Department of Genetics at Stanford.  Isolation of genes and enzyme activities involved in DNA repair and/or recombination in Bacillus subtilis.

June 1979-May 1980:  Undergraduate honors thesis in the laboratory of Dr. William Steinhart in the Biology Department at Bowdoin College.  Effect of Herpes Simplex Virus (Type 1) infection on phospholipid metabolism of human tissue culture cells.

Summer 1976: ACS fellowship to work in the surgical research laboratory of Dr. William Sharp at Akron City Hospital in Akron, Ohio.

Memberships:

Association of Biomolecular Resource Facilities (survey Committee member since 1999)

American Association for the Advancement of Science

American Society for Microbiology.

Publications:

1.  Steinhart, W.L., Nicolet, C.M., and Howland, J.L. (1981) Incorporation of 32P-phosphate into membrane phospholipids during infection of cultured human fibroblasts by Herpes Simplex Virus Type I.  Intervirology 16: 80-85.

2.  Nicolet, C.M., Chenevert, J.M., and Friedberg, E.C. (1985) The RAD2 gene of Saccharomyces cerevisiae:  nucleotide sequence and transcript mapping.  Gene 36: 225-234.

3.  Robinson, G.W., Nicolet, C.M., Kailanov, D., and Friedberg, E.C. (1986) A yeast excision repair gene is inducible by DNA damaging agents.  PNAS 83:  1842-1846.

4.  Fleer, R., Nicolet, C.M., Pure, G., and Friedberg, E.C. (1987)  RAD4 gene of Saccharomyces cerevisiae:  molecular cloning and partial characterization of a gene that is inactivated in E. coli. Mol. Cell. Biol. 7:  1180-1192.

5.  Nicolet, C.M. and Friedberg, E.C. (1987) Overexpression of the RAD2 gene of S. cerevisiae:  Identification and preliminary characterization of Rad2 protein.  Yeast 3:  149-160.

6.  Craig, E.A., J. Kramer, J. Shilling, M. Werner-Washburne, S. Holmes, J. Kosic-Smithers, and C.M. Nicolet (1989)  SSC1, an essential member of the yeast HSP70 multigene family, encodes a mitochondrial protein.  Mol. Cell. Biol. 9:  3000-3008.

7.  Nicolet, C.M. and E.A. Craig (1989) Isolation and characterization of STI1, a stress-inducible gene from Saccharomyces cerevisiae.  Mol. Cell. Biol. 9:  3638-3646.

8.  Bardwell, L., H. Burtscher, W.A. Weiss, C.M. Nicolet, and E.C. Friedberg (1990)  Characterization of the RAD10 gene of S. cerevisiae and purification of Rad10 protein.  Biochemistry 29:  3119-3126.

9.  Nicolet, C.M. and E.A.Craig (1991) Functional analysis of a conserved amino terminal region of hsp70 using site-specific mutagenesis.  Yeast 7:  699-716.

10. Wynn, T.A., C.M. Nicolet and D.M. Paulnock (1991).  Identification and characterization of a new gene family induced during macrophage activation.  J. Immunol 147:  4384-4392.

11. Nelson, R.J., T. Ziegelhoffer, C.M. Nicolet, M. Werner-Washburne, and E.A.Craig (1992).  The translation machinery and 70 kd heat shock protein cooperate in protein synthesis.  Cell 71:  97-105.

12. Nicolet, C. M. and D. M. Paulnock (1994).  Promoter analysis of an interferon-inducible gene associated with macrophage activation.  J. Immunol. 152: 153-162.

13. McDowell, MA, DM Lucas, CM Nicolet and DM Paulnock (1995).  Differential Utilization of IFN-gamma responsive elements in two maturationally distinct macrophage cell lines.  J. Immunol 155: 4933-4938.

14. Nicolet, CM, Burkholder, JK, Gan, J, Culp, J, Kashmiri, SVS, Schlom, J, Yang, N-S, and Sondel, PM (1995).  Expression of a tumor-reactive antibody-IL2 fusion protein following in vivo particle mediated gene delivery. Cancer Gene Therapy 2: 161-170.

15. Nicolet, C.M., Siegel, D.H., Surfus, J., and Sondel, P.M. (1997).  TAG-72 reactive antibody CC49 recognizes molecules expressed by hematopoietic cell lines.  Tumor Biology 18: 356-366.

16. Nicolet, CM, Surfus, JA, Hank, JA, and Sondel, PM. (1998).  Transcription factor activation in lymphokine activated killer cells and lymphocytes from patients receiving IL-2 immunotherapy.  Cancer Immunol Immunother 46: 327-337.

17.   McMillen, DA, Old, WM, Bleibaum, JL, Nicolet, CM, Resing, KR, Sarath, G, and Yadav, SP (2000).  Job compensation in the biotechnology core laboratory.  Nature Biotechnology 18 (6):  686-689.

Book Chapters:

1.  Friedberg, E.C., Barbis, D.P., Chenevert, J.M., Fleer, R., Kailanov, D., Naumovski, L., Nicolet, C.M., Robinson, G.W., Schultz, R.A., Weiss, W.A., and Yang, E. (1985) Molecular Approaches to the Study of Nucleotide Excision Repair in Eukaryotes. In Mechanisms of DNA Damage and Repair  M.G. Simic, C. Grossman, and A. Upton (eds.).

2.  Friedberg, E.C., Fleer, R., Naumovski, L., Nicolet, C.M., Robinson, G.W., Weiss, W.A., and Yang, E. (1985)  Nucleotide Excision Repair Genes from the Yeast Saccharomyces cerevisiae.    In Mechanisms of Antimutagenesis and Anticarcinogenesis  P. Hartman, T. Kada, and A. Hollaender (eds.).

3.  Friedberg, E.C., Cooper, A.J., Couto, L., Fleer, R., Naumovski, L., Nicolet, C.M., Robinson, G.W., and Weiss, W.A. (1987) Yeast as a eukaryotic model for the study of nucleotide excision repair.  In DNA Repair:  A Laboratory Manual of Research Procedures, Volume 3  E.C. Friedberg and P.C. Hanawalt (eds.).

4.  Friedberg, E.C., A.J. Cooper, L. Couto, R. Fleer. D. Kailanov, L. Naumovski, C.M. Nicolet, J. Rehm, G. W. Robinson, B.H. Ryu, T. Sugino, W.A. Weiss, and E. Yang (1987)  Recent advances in the characterization of genes and proteins for nucleotide excision repair in the yeast S. cerevisiae.  Photochem. Photophysics. 1987 (Suppl.):  333-341.

5. Craig, E.A., W. Boorstein, H.O. Park, D. Stone, and C.M. Nicolet (1989)  Complex  regulation of three heat inducible HSP70 related genes in Saccharomyces cerevisiae. In Stress-Induced Proteins  M.L. Pardue, J.R. Feramisco, and S. Lindquist (eds.).

6.   Nicolet, C.M. and E.A. Craig (1991)  Inducing and assaying the heat shock response in Saccharomyces cerevisiae.  Methods in Enzymology 194:  710-717.

7.    Nicolet, C.M. and N.-S. Yang (2000).  The use of particle mediated gene transfer for the study of promoter activity in somatic tissues.  Methods in molecular Biology 130:103 (Humana Press).